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Maxima SYBR Green/ROX qPCR Master Mix (2X), 4000 Reactions

Manufacturer: Thermo Fisher Scientific
Availability: in stock 0 item(s)
Product Code: P2094589
SKU: K0223
UOM: 4000 reactions
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Product Sub Group: Sybr Green Mastermix

Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (see Resources for a compatibility chart).

Highlights
• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2x master mix

Applications
• Gene expression
• siRNA validation
• Genotyping
• Pathogen detection

Includes
Maxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.

For more details, please follow https://www.thermofisher.com/order/catalog/product/K0223?SID=srch-hj-K0223


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