Retroviral Mammalian Expression Kit
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ III – Retroviral incorporates the Human elongation factor 1 alpha promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method and also, it can be enriched from negative clones with magnetic beads bearing an antiΔNGFR antibody
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